PCR Reagents & Enzymes
PCR Reagents & Enzymes
PCR product
PCR (Polymerase Chain Reaction) is a widely used molecular biology technique for amplifying DNA sequences. Several product types are essential for performing PCR effectively, including reagents, enzymes, and kits for various stages of the process. Here's a list of key product types:
PCR Reagents & Enzymes
DNA Polymerases: Essential for synthesizing the new DNA strand during PCR. Examples include:
Taq Polymerase: A heat-stable enzyme from Thermus aquaticus, widely used in standard PCR.
Pfu Polymerase: A high-fidelity enzyme for more accurate DNA amplification, often used in applications requiring minimal errors.
dNTPs (Deoxynucleotide Triphosphates): These are the building blocks (A, T, C, G) that the polymerase uses to build the new DNA strands.
PCR Buffers: Buffer solutions that provide the necessary ionic strength and pH for optimal enzyme activity.
PCR Master Mix Kits: Pre-mixed solutions that contain the enzyme, dNTPs, and buffer in the correct proportions for PCR. They simplify the setup process and reduce the chance of errors.
Hot-Start PCR Kits: PCR kits that include a heat-activated polymerase to prevent premature amplification, which can occur at lower temperatures.
High-Fidelity PCR Kits: These kits contain enzymes optimized for greater accuracy in DNA amplification, with minimal errors in the amplified product.